Supporting Information for MEDYAN: Mechanochemical Simulations of Contraction and Polarity Alignment in Actomyosin Networks

Reaction Symbol Value [for simulation] Reference Actin diffusion kactin, diff 20 μm 2 · s−1 [80 s−1] a α-actinin diffusion kα,diff kactin, diff/10 s −1 a NMIIA mini-filament diffusion kNMIIA, diff kactin, diff/100 s −1 a Actin polymerization at plus end kactin,poly,+ 11.6 μM −1s−1 [0.151 s−1] b [1] Actin polymerization at minus end kactin,poly,− 1.3 μM −1s−1 [0.017 s−1] b [1] Actin depolymerization at plus end kactin,depoly,+ 1.4 s −1 [1] Actin depolymerization at minus end kactin,depoly,− 0.8 s −1 [1] NMIIA head binding kNMIIA,bind 0.2 s −1 [2] NMIIA head unbinding under no external load k NMIIA,unbind 1.7 s −1 c α-actinin binding kα,bind 0.7 μM −1s−1 [0.009 s−1] [3] α-actinin unbinding under no external load k α,unbind 0.3 s −1 [3]